Fluorescence Spectrophotometer Laser Spectroscopy

Spectrophotometry yog ib txoj hau kev ntawm kev tsim nyog thiab ntsuas kev ntsuam xyuas ib yam khoom los ntawm kev ntsuas qhov nqus ntawm lub teeb ntawm ib lub qhov ntev ntawm cov kabmob lossis ib tus kabmob qoob loo. Nws muaj qhov zoo ntawm kev siab zog, kev lag luam yooj yim thiab kev ceev, thiab feem ntau yog siv cov kev sim hauv cov kev sim biochemical. Ntau yam tshuaj yog ntsuas los ntawm spectrophotometry. Hauv ib lub spectrophotometer, thaum lub teeb ntawm cov wavelengths sib txawv tas li tsis tu ncua mus rau ib qho piv txwv ntawm kev qee concentration, kev nqus siv sib xyaw sib haum mus rau lwm lub wavelength tau.

Cov cim tseem ceeb uas cuam tshuam cov kev sim

Wavelength qhov tseeb thiab repeatability

Txhua tus nqi ntawm qhov ntsuas yog ntsuas ntawm ib lub suab qis. Yog hais tias tus wavelength tau tshwm sim thiab qhov tseeb wavelength tig los ntawm ib plhom mais, ces qhov kev sib tw ntawm qhov ntsuas thiab tus nqi muaj tseeb yog dab tsi? Qhov tseem ceeb ntawm qhov ntsuas no tuaj yeem pom. .

Txij nkawm thiab kho

1. Monochromator. Lub monochromator yog qhov tseem ceeb ntawm qhov ntsuas thiab muaj tsev nyob hauv ib lub thawv ntim thiab tsis tuaj yeem ua dej num. Qhov xaiv lub wavelength yuav tsum tau tig rau hauv ib qho kev ncaj ncees, thiab qhov tsis muaj peev xwm muaj zog dhau lawm. Yuav kom tiv thaiv tau cov ntsiab ntawm cov ntsiab lus ntawm cov dej ntws, cov monochromator desiccant (silica gel) yuav tsum tau hloov lub sijhawm. Yog hais tias tus txiv laej tawm tau pom tias nws muaj qhov sib txawv, nws yuav tsum hloov tam sim ntawd.

2. Pas dej txias. Lub nqus ntawm tes yuav tsum tau siv kom raug thiab tshwj xeeb kev tu xyuas yuav tsum tau coj los tiv thaiv ob lub ntsej muag muag ntawm lub nqus dej.

3. Ntes. Lub tshuab hloov dua siab tshiab yuav tsis tuaj yeem raug rau ntev, thiab yuav tsum tsis txhob muaj lub teeb ntawm lub teeb lossis lub cev ntub dej.

4. Thaum lub tshuab nres tsis ua haujlwm, lub hwjchim yuav tsum muab tua.

5. Kom tsis txhob muab cov plua plav thiab cov kis kab mob pov tseg, npog cov hmoov av npog thaum koj nres hauj lwm.

6. Yog tias qhov ntsuas tsis tas ua kom muaj zog, nws yuav tsum tsis pub tsawg tshaj 20 ~ 30min txhua lub sijhawm kom lub tshuab tag nrho kom qhuav thiab tswj cov khoom siv hluav taws xob.