Cov Molecules tuaj yeem txiav ib yam nkaus

Cov molecules puas tuaj yeem raug txiav thiab? 

Nrog rau txoj kev loj hlob thiab kev loj hlob ntawm cov tiam tom ntej sequencing (NGS) technology, daim ntawv thov muaj nyob rau hauv ntau yam lag luam kev lag luam ntawm kev tshawb fawb, kev kuaj mob, thiab kev kuaj lub cev tau txuas ntxiv mus. Niaj hnub no, nrog rau kev txhim kho tas mus li ntawm kev ua lag luam, kev loj hlob ceev ntawm sequencing thev naus laus zis nws tus kheej tau maj mam qeeb, thiab cov txiaj ntsig ntawm cov qauv kev npaj hauv NGS tau dhau los ua pom tseeb dua. Nyob rau tib lub sijhawm, cov txheej txheem tshiab uas tsom mus rau qhov ua tiav me me ntawm cov qauv raug tsim los tas li, tab sis DNA tawg yog ib txwm ib qho kev zam tsis tau hauv cov txheej txheem ntawm NGS lub tsev qiv ntawv kev tsim kho.

Ultrasonic fragmentation, raws li hydrodynamic shearing thiab ultrasonic fragmentation, yog feem ntau txoj kev ntawm DNA fragmentation. Lub hauv paus ntsiab lus yog: nyob rau hauv qhov kev txiav txim ntawm high-frequency pulses, cov kua generates countless high-pressure point thiab low-pressure point, thiab cov accompanying cavitation npuas nyob rau hauv ob peb milliseconds thiab tawg vim lub siab hloov. Hauv cov txheej txheem no, instantaneous high-strength shear yog tsim nyob rau hauv cov kua. quab yuam, uas tuaj yeem rhuav tshem cov hlwb, cuam tshuam rau cov protein, thiab txiav DNA. Nov yog cov khoom tshwj xeeb ntawm Xinzhi Bio: SCIENTZ18-A Ultrasonic DNA Interruption Instrument, uas siv txoj kev ua haujlwm tsis sib cuag thiab tuaj yeem ua tiav txog 12 cov qauv hauv ib zaug. Nws yog chromatin immunoprecipitation (Chromatin Immunoprecipitation , CIP) thiab cov cuab yeej txheem rau DNA shearing kev tshawb fawb platforms.