Daim Ntawv Thov Ntawm Cov Paj Tsuag Hauv Cov Qoob Hauv Qhov Kev Ua Rau Hauv Cov Qos Ib Ncauj Sweet

Pig Yim Rings: Tus txiv neej liab qab cawm kuv!

Wukong: nerd, dab tsi yog flustered!

Pig Yim Rings: Monkey Brother, koj tsis tau hnov ntawm nws! Cov npua neeg Asmeskas npua tuaj, muaj ib tus kab mob, thiab ib lub zes muaj mob! Kuv tau hnov hais tias ntau tus kwv tij nyob hauv Liaoning, Heilongjiang, Jilin, Jiangxi, Fujian, thiab Sichuan tau raug culled.

Wukong: nerd! Qhov no tsis yog deb ntawm kuv!

Nqe lus nug 1. Tus kab mob African npaws yog dab tsi?

Neeg Asmeskas Swine Fever (ASF) yog ib yam kab mob ntsws, mob hnyuv, thiab mob hnyav heev los ntawm cov kab mob npaws Neeg Asmeskas Swine Fever (ASFV) ntawm cov npua hauv npua thiab lwm yam kab mob qus (tsis kis kab mob). Nws yog qhov pib los ntawm lub sijhawm luv luv, nrog rau kev tuag ntawm 100% rau cov kab mob tsis meej thiab mob hnyav, thiab tsuas pom tseeb los ntawm kev kuaj xyuas.

Lub Yim Hli 3, 2018, Tuam Tshoj tau pom zoo thawj rooj plaub ntawm African swine fever.

Nqe Lus Nug 2: Taw qhia rau cov qauv kev ua piv txwv rau cov kab mob swine flu rau African

I. Piglet Piv txwv kev coj ua

1. Lub hom phiaj

Txhim kho cov txheej txheem rau ASFV cov qauv thiab cov ntaub ntawv sim kom paub meej txog cov kev sim ntawm ASFV txog kev sim thiab biosafety hauv chaw soj ntsuam.

2. Caw ntawm daim ntawv thov

Kev sim ua ntej rau kev kho mob ASFV kuaj kab mob

3. Tus txheej txheem

3.1 Cov khoom siv

ASF raug cov tsiaj txhu, feem ntau yog cov npua ntawm cov tsiaj qus, cov qus npua thiab cov kooj, feem ntau sau cov qauv ntawm cov npua hauv npua, cov qus npua cov qog, cov qauv nqaij thiab cov kab laib. Cov qauv hauv qhov kev ntsuam xyuas yuav tsum tau ua ntej kev kho mob yooj yim rau kev kuaj mob thiab kev kuaj mob.

Ntshav: rau kev kis tus kab mob nucleic acid, viral antigen thiab antibody nrhiav kom tau. Tus kabmoos (syringe) los yog cov ntshav sau los ntawm cov ntshav uas tau muab coj los tso rau ntawm qhov chaw sov li 2 rau 4 teev (tiv thaiv tau), lossis muab tso rau hauv 37 ° C incubator rau 1 h. Tom qab cov ntshav qab zib feem ntau yog precipitated, cov ntshav qab zib tawm, thiab cov ntshav tau sib cais los ntawm centrifugation yog tias tsim nyog. . (Pom zoo 5 ml).

Anticoagulated tag nrho cov ntshav: siv rau kev kis tus kab mob nucleic acid thiab kev kis tus kab mob viral antigen.

Tus kab mob anticoagulant tau muab tso ua ke los ntawm cov hlab ntaws ntshav los yog lub raj yas ua ntej tso ntshav. Sau cov ntshav thiab maj mam txaij nws rov qab los sib tov cov ntshav thiab anticoagulant los tiv thaiv cov ntshav tawm ntawm cov ntshav thaum tsis txhob hemolysis. Nws tseem ua tau kom cov ntshav tso rau hauv ib lub raj mis uas muaj lub khob fais fab thiab muaj co tawm fibrin. Yog tias tsim nyog, ib ob koob tshuaj tiv thaiv kab mob yuav muab ntxiv rau inhibit kab mob sib kis kab mob uas tshwm sim thaum lub sij hawm cov ntshav los yog cov ntshav sau.

Thaum kuaj ntshav siv rau kev kis tus kab mob nucleic acid, EDTA feem ntau yog siv los ua ib qho anticoagulant uas tsis muaj heparin. (Qhov pom zoo tsawg kawg 1mL).

Cov ntaub so ntswg: nyob hauv vivo sau, siv cov hnyuv, rau kev saib xyuas kev noj qab haus huv ASF xyuas.

Autopsy ASF raug tua los ntawm cov tsiaj los yog tuag cov tsiaj raug muab pov tseg, xws li: ASFV lub plab los ntawm cov khoom plab: spleen, lymph nodes, raum, tonsils.

Sau los ntawm lwm yam kabmob ntsaj xws li mob siab ntsws xws li daim siab, lub ntsws, lub plawv, thiab lwm yam kev pom zoo, thiab xaiv lub tsev kawm ntawv ntawm cov kabmob ntawm cov kabmob thiab cov ntaub so ntswg noj qab nyob zoo.

Sample collection of corrupted animal carcasses: Lub femur raug coj los ntawm necropsy, thiab cov leeg nqaij thiab ligaments txuas tag nrho tawm, thiab pob txha pob txha tau sau. Tus nqi tsawg yog 5 grams.

Tus kab laum yog ua los ntawm cov kab laib: cov kab laib yuav tuaj yeem nqa tawm ntawm tus kabmob nucleic acid, kuaj pom tus kabmob thiab kuaj kabmob (morphological identification of cockroaches, nrhiav phab ntsa, phab ntsa, ntoo lossis vov tsev ntawm npuaj npuaj, lossis nrhiav kab laum nyob hauv av npua npua, los yog saib cov npua Epidermis, khaws cov hnoos qeev.

Ceebtoom: Cov qauv siv rau kabmob cais thiab kev kuaj kab mob yog txwv tsis pub cia ntawm-20 ° C.

3.2 Cov cuab yeej siv thiab cov tshuaj reagents

1) Duab lag luam high-speed tub yees centrifuge, ntaub qhwv zeeg, dej da dej, timer, tub yees thiab kav dej.

2) Txiab txiab, lub plawv, hlau pob, 2 mL EP tub, tswv yim xa xov (10 μL, 200 μL, 1000 μL), thiab lwm yam.

3) Lwm yam reagents thiab consumables xws li: 0.01mol / L PBS (pH 7.2), 0.8% NaOH, 75% cawv paj rwb, thiab cov zoo li.

3.3 Cov Qauv Ua Haujlwm

3.3.1 Ntshav thiab kuaj ntshav tag nrho

Noj 1 mL ntawm cov ntshav dej los sis cov ntshav txhij txhua hauv lub raj ntawm EP tub thiab txuag siv.

3.3.2 Tissue sample processing

Nws yog haum rau kev kho mob ntawm cov ntaub so ntswg xws li tus poov thiab hnoos qeev. Txo cov pa phem kom ntau li ntau tau. Rau txhua lub pob khaus los yog hnoos qeev, siv ib qho khoom siv piv txwv xws li cov nplaim hluav taws txiab. Cov ntaub so ntswg yuav tsum muab tso rau hauv ib lub thawv uas muaj sterilized thiab khi tam sim ntawd, thiab cim kom tiv thaiv kev sib kis ntawm cov nqaij.

3.3.2.1 Kev sib tw ntawm cov ntaub so ntswg yuav tsum tau ua nyob rau hauv ib lub tsev txeeb qib biosafety. Tsis txhob cia cov dej ntws mus rau hauv lub txee rau lub cev.

3.3.2.2 Muab 0.1-0.2g ntaub so ntswg thaiv rau hauv 2mL raj, txiav rau txiab nrog txiab, ntxiv 1-2mL 0.01mol / L PBS (pH 7.2), tom qab ntawd ntxiv 1-2 sterilized steel npas, tom qab sealing Tus nplaum yog tshuaj tua kab mob rau kev siv. Cov hnyuv ntxiv rov qab mus rau lub thauv, rov ua dua tshiab dua, thiab cov nplaum yog sterilized thiab muab cia rau hauv -70 ° C yees.

3.3.2.3 Fragmentation of tissue samples Cov ntaub so ntswg yog muab ntxig rau sab nraud ntawm lub txee rau lub cev kom ua rau cov ntaub so ntswg mus rau hauv cov nqaij mos.

Hnyavyweight pom zoo kom kev tshawb fawb ntawm Scientz-48L uas muaj zog hauv cov ntaub so ntswg

3.4 Tus kab mob tsis zoo

Qhov inactivation ntawm tus kab mob no yuav tsum tau nqa tawm nyob rau hauv ib tug secondary biological kev ruaj ntseg txee. Cov ntshav qab zib, tag nrho cov ntshav los sis cov av thiab cov ntaub so ntswg tawg tau muab tso rau hauv 60 ° C dej da dej ua ke nrog lub raj xa dej EP thiab tso cai rau sawv ntsug rau 30 min rau kev pab.

3.5 Ua ntsuas tom qab siv

Txiab, caws pliav, thiab lwm yam yuav tsum tau muab tso rau hauv lub khob kom tsis muaj kab mob, muab tso rau hauv lub plawv su noj, thiab muab tso rau hauv ib lub hnab ntim rau cov khoom tsis zoo. Lub thauv uas muaj cov ntaub so ntswg qauv txuag kua thiab lub centrifuge tube uas muaj cov ntaub so ntswg yuav tsum muab ntim, thiab cov nplaum yog sterilized thiab muab tso rau hauv ib lub hnab ntim rau autoclaving. Cov npas hlau, cov tswv yim thiab lwm yam qias neeg raug muab tso rau hauv 0.8% NaOH rau 30 feeb, thiab tom qab ntawd muab tso rau hauv ib lub hnab ntim rau autoclaving. Cov tshuaj tua kab mob yuav tsum tau autoclaved hauv lub thawv ntim.

3.6 Cov cwj pwm

Ua ntej kev khiav hauj lwm, tshawb xyuas tias cov khoom siv roj ntsha thiab kuaj sim tau zoo kom huv si los tiv thaiv kev kis kab mob ntawm cov qauv.

Thib ob, cov nucleic acid detection ntawm tus kab mob swine flu npua Neeg Asmeskas tuaj yeem nqa tau los ntawm PCR lub sijhawm, thiab cov kauj ruam ua haujlwm yuav raug coj mus rau cov kev qhia ntawm lub tuam txhab kev tsim khoom.

Nqe lus nug 4, tus kab mob tua kab thiab kev tiv thaiv thiab kev tswj kev ua haujlwm

Txawm hais tias tus kab mob npaws neeg Asmeskas hauv tus kab mob npaws yog nyob ruaj khov hauv ib puag ncig, nws yuav yooj yim rau tus neeg tsis noj nqaij thaum lub sijhawm ua noj nqaij npuas. Thaum sov 70 ° C-75 ° C rau ntau tshaj 30 feeb, tus kabmob yuav raug tua, uas txhais tau hais tias peb tsuas xav tau noj thaum lub caij ua noj txhua hnub. Noj cov nqaij npuas kom huv si (nrog rau cov cua txias hauv nruab nrab ntawm qhov kawg ntawm 70 ° C), txawm tias muaj ib tus kab mob yuav ua rau kom tsis muaj peev xwm kis tus kab mob thiab ua poob haujlwm.

Kev Taw Qhia Txog Suab Paj Nruag Bio Sciencez-48L Cov Khaub Ncaws Ntawm Cov Quav Ntaub Ntawv

Lub Scientz-48L khov high-throughput cov ntaub so ntswg los ntawm Shinzo Bio muaj cov nram qab no nta:

1. Ntau yam kev hloov scenarios. Cov ntaub so ntswg sib txawv tau qhuav qhuav-grinded, ntub dej-milled , tov , thiab homogenized .

2. Kev sim yog ib qho zoo heev. Nws tuaj yeem txhim kho kom zoo dua qub thiab nucleic acids uas muaj ntau qhov hnyuv.

3. Txoj kev sim yog qhov zoo. Kev sib tsoo txoj kev siv siv lub raj siv centrifuge uas raug kaw, uas ua rau kom tsis txhob kis tus kabmob ntawm tus qauv.

4. Qhov ntsuas tau ruaj ntseg zoo. Qhov kev sib tsoo tib yam yuav tsum tau teem rau tib cov ntaub so ntswg kom ua tiav qhov kev sib txuam zoo tib yam thiab ua kom zoo dua qhov kev sim ua dua.

5. Cov nyhuv sib txig yog qhov zoo. Cov khoom yog hauv av nrog kev sib tsoo cov khoom sib tsoo, uas muaj ntau dua thiab txaus sib tsoo cov qauv piv rau cov qauv kev sib tsoo.

Tam sim no, scientz Biotechnology Scientz-48L frozen high-flux daim ntaub liam tau pab Shenyang, Dalian, Chaoyang, Benxi, Anshan, Jinzhou, Huludao thiab lwm yam tsiaj kab mob kev tswj cov qauv rau kev ua piv txwv ntawm cov npua Neeg Asmeskas swine fever sim kev sim. .

Kev ntsuas siab, ruaj khov thiab ruaj ntseg zoo ntawm Sciencez-48L frozen high-flux daim ntaub pob zeb zoo li daws cov teeb meem ntawm ntau cov qauv nyob rau hauv txoj kev paub thiab qhov teeb meem ntawm kev tswj cov kev sib thooj ntawm cov kev sim, uas tau zoo tau txais los ntawm cov neeg siv.

Khoom Siv

1. Tsim kom muaj kev sib tsoo thiab crushing ntau hom ntaub so ntswg xws li cov hauv paus hniav, kab tshoob, nplooj, paj, txiv hmab txiv ntoo, noob, thiab lwm yam. 2. Haum rau ntau cov tsiaj cov nqaij nrog rau lub hlwb, lub plawv, mob ntsws, lub plab, lub siab, cov thymus, crushing ntawm cov qog cov leeg, cov leeg, cov pob txha, thiab lwm yam .; 3. Tsim kom muaj kev sib tsoo thiab tsoo cov kab mob hu ua fungi, bacteria thiab lwm yam; 4. Kev sib tsoo thiab crushing rau cov khoom noj khoom haus thiab cov khoom siv tshuaj; 5. Tsim nyog rau cov neeg tsis muaj zog xws li cov roj thiab cov roj sib tsoo thiab kev sib tsoo ntawm kev kuaj xws li cov khoom siv roj thiab cov khoom siv quav muag; 6. Kev tsim kom sib tsoo thiab crushing ntawm plastics, polymers nrog PE, PS, textiles, resins thiab cov zoo li.

Lub ntsiab technical tsis

1. Lub sijhawm teev: 1 (vib nas this) - 9999 (seconds)

2. Kev ncua sij hawm: 10-70Hz, uas yog, oscillation 300-2100 zaug / min

3. Fais fab tuag: 180W

4. Fixture mob stroke: 34mm (ntsug)

5. Kub qhov chaw: -20 ° C los -30 ° C

6. Electromagnetic lock control opens lub qhov rooj

7. Cov qauv peev txheej: txheej txheem txhim khu: txhuas txhuas adapter 5ml 12 qhov, 2 ml 48 qhov

Yeem: Aluminium adapter 2ml24 qhov

8. Cov yuav tsum tau muaj zog: 220V ib zaug xwb AC, <>

Science lub tuam txhab biotechnology profile

Ningbo scientz biotechnology co., Ltd (Tshuag code: 430685) yog tsim los ntawm 1989. Lub tuam txhab yog headquartered hauv National High-tech Zone ntawm Ningbo, nrog ib tug peev ntawm peev ntawm 61.02 million yuan. Nws yog ib lub ntiaj teb-nrov npe ntawm cov khoom siv roj ntsha cov qauv ua haujlwm thiab ib daim ntawv thov siv ultrasonic technology. Lub forerunner ntawm kev tshawb nrhiav.

Lub tuam txhab tam sim no muaj plaub R & D cov chaw hauv Ningbo thiab Hangzhou, nrog ntau cov neeg ua haujlwm R & D cov neeg ua haujlwm thiab ntau dua li ntawm 60 daim ntawv pov thawj.

Lub tuam txhab muab qhov tseem ceeb rau kev cog qoob thiab kev ua lag luam. Yuav kom tau txais kev pab zoo dua qub, lub tuam txhab muaj ntau tshaj 30 chaw thoob plaws hauv lub tebchaws. Cov chaw khiav dej num muaj cuab yeej txuas nrog engineers kev muag khoom thiab tom qab muag muag engineers kom ua raws li cov neeg xav tau txhua lub sijhawm. Ntau tshaj 15,000 lub chaw soj ntsuam nyob thoob ntiaj teb siv Xinzhi Bio cov khoom, thiab lub khw rau ntau yam twj paj nruag thiab khoom siv ntau tshaj 100,000 leej. Cov khoom muag raug xa tawm mus rau 36 lub teb chaws xws li Teb Chaws Asmeskas, Tebchaws Asmeskas, Fab Kis, Russia, Nyiv thiab Kauslim, thiab tau los ua neeg thiab thoob ntiaj teb. Ib tug paub zoo kws nyob rau hauv kev loj hlob ntawm kev lom khoom lom cov khoom.