Host cell residual DNA cov qauv npaj
May 21, 2021
Tso lus
Recombinant protein tshuaj, tshuaj tiv thaiv kab mob, tshuaj tiv thaiv kab mob thiab lwm yam khoom siv hauv cov khoom siv roj ntsha tau qhia thiab tsim los ntawm cov kab txuas ntxiv ntawm tes. Txawm hais tias lawv tau ua dhau los ntawm cov txheej txheem kev ua kom huv si, tseem yuav muaj cov khoom seem DNA ntawm cov khoom tawg (rDNA) hauv cov khoom lag luam.
Cov rDNA no yuav cuam tshuam rau kev ua haujlwm ntawm cov tseem ceeb ntawm tus txais, yog li nqa txoj kev pheej hmoo kis lossis qog nqaij hlav.
Yog li, yuav ua li cas thiaj tau txais txiaj ntsig thiab tau txais qhov cim tseg rDNA hauv qhov piv txwv yog qhov tseem ceeb. Ntawm nimno, muaj ntau txoj kev hauv qab no:
| Txoj kev | Cov lus txhawb zoo | Nyias tsis zoo |
| Phenol / Chloroform | High DNA purity thiab cov ntsiab lus siab | Ua lub sijhawm ua haujlwm, txiav nyom, thiab muaj tshuaj lom |
| Sodium iodide dej nag lossis daus | yooj yim siv | Siv sijhawm thiab tsis txaus ntseeg cov DNA |
| Txoj kev qhia kiag | Yooj yim thiab siab purity | Siv sijhawm thiab nyuaj rau automate |
| Sib Nqa Cov Nqaj Siv Hluav Taws Xob | Yooj yim, siab purity, tuaj yeem siv tshuab | - |
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Cov txheej txheem kev kho mob no yog ua raws li cov hauv paus ntsiab lus ntawm cov hlau nplaum sib nqus cov qauv thiab siv nyob rau hauv rDNA pretreatment system ntawm cov khoom siv roj ntsha, uas tuaj yeem paub txog cov kev ua tau zoo, yooj yim thiab tsis siv neeg ua haujlwm.
Lub SCIENTZ tsis siv neeg nucleic acid rho tawm cov cuab yeej muaj cov khoom lag luam muaj npe zoo ntawm cov tswv tsev ntawm cov khoom seem DNA coj los kuaj cov khoom siv (cov qauv sib nqus), thiab tus nqi rov qab ntawm ntau cov qauv yog ruaj khov ntau dua 80%, uas tuaj yeem ruaj khov thiab tswj tau. lub host cell DNA cov ntsiab lus nyob hauv ntau yam khoom siv roj ntsha
Xyaum ua haujlwm pabcuam
Cov khoom siv sim: tsis siv neeg nucleic acid rho tawm ntsuas (NP-2032), ntsuas fluorescent kom muaj ntau qhov ntsuas PCR (LightCycler480), cov tswv tsev ntawm cov seem DNA ntawm cov khoom siv pretreatment (sib nqus hlaws txheej txheem), CAR / TCR noob luam cov naj npawb nrhiav cov khoom siv (multiplex PCR- Fluorescence probe method) )
Cov qauv sim: cov qauv ntsuas kabmob, cov qauv CAR-T ntawm tes, kev tswj tsis zoo
Cov kauj ruam sim:
Txog kev tshuaj xyuas.
Kev sim ua piv txwv-npaj npaj-rho ntawv npaj-qhov kev pab pib-tag nrho cov ntsiab lus kuaj pom
Ceeb toom: Qhov ntsuas sau txog li 30 feeb
Cov txiaj ntsig sim:
| Qauv lub npe | Lub ntsiab lus | MeanConc | Nyiaj tau rov qab los | Caws pliav | Tus nqi rov qab |
| Kev tswj tsis zoo | 36.43 | 0.00 | - | - | - |
| Kev tswj tsis zoo + (portable) | 18.67 | 86.62 | 8662.15 | 9707.85 | 89% |
| Kev tswj tsis zoo | 18.72 | 53.47 | 8020.723657 | 9868.821045 | 81% |
| Tus qauv kab mob + | 19.15 | 63.10 | 8194.27 | 9707.85 | 84% |
| Tus qauv ntawm tes | 20.21 | 32.93 | 2811.241943 | 2740.516576 | 102% |
Cov txiaj ntsig sim:
Kev siv hluav taws xob tsis zoo ntawm cov qauv sib txawv muaj cov txiaj ntsig ruaj khov thiab ua tiav;
Batch ua haujlwm rau kev siv hluav taws xob ua haujlwm siab dua cov kev siv tes rho tawm;
Nws ua tau raws li qhov yuav tsum ua ntawm USP cov nyiaj rov qab tshiab; nws tuaj yeem yog cov neeg pabcuam zoo rau kev npaj ua ntej ntawm rDNA kuaj pom nyob rau hauv cov khoom siv roj ntsha.
Cov khoom lag luam:
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